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銆�銆�鏃ュ墠錛岀敱寮楅浄寰峰搱閽︽．鐧岀棁鐮旂┒涓績(jī)(Fred Hutchinson Cancer Research Center)棰嗗鐨勪竴涓浗闄呯爺絀跺皬緇勮瘉瀹炰簡(jiǎn)澶ц妯°�佹爣鍑嗗寲铔嬬櫧璐ㄦ嫻嬬殑鍙鎬э紝榪欐槸楠岃瘉鐤劇梾鐢熺墿鏍囧織鐗╁拰鑽墿闈剁偣鐨勫繀瑕佹潯浠躲�傝繖欏瑰垔鐧誨湪銆婅嚜鐒?鏂規(guī)硶銆?Nature Methods)鏉傚織涓婄殑鏈�鏂拌鏂囪〃鏄庯紝縐戝瀹朵滑寮�鍙戠殑涓�縐嶉澏鍚戞�ц泲鐧借川媯�(gè)嫻嬫柟娉曞叿鏈夌郴緇熷湴銆佸彲闈犲湴媯�(gè)嫻嬩漢綾昏泲鐧借川緇勭殑娼滃姏銆?/span>

銆�銆�璁烘枃涓昏浣滆�呫�佺檶鐥囪泲鐧借川緇勫涓撳 Amanda Paulovich 鍗氬＋鍜屽悓浜嬩滑寮�鍙戠殑榪欓」鎶�鏈紝鍙悓鏃跺噯紜湴媯�(gè)嫻嬭澶氫笉鍚屾牱鏈腑鎴愮櫨涓婂崈縐嶈泲鐧借川鐨勪赴搴︺�傛潵鑷タ闆呭浘銆佹嘗澹】鍜岄煩鍥界瓑鍏朵粬鍦板尯鐨勫疄楠屽閲嶇幇浜?jiǎn)錆h綾諱鉤鑵虹檶緇嗚優(yōu)涓?319 縐嶈泲鐧借川鐨勬嫻嬬粨鏋滐紝璇佸疄榪欑鏂規(guī)硶鍙法瓚婂疄楠屽鍜屽浗鐣屽疄鐜版爣鍑嗗寲銆?/span>

銆�銆�Paulovich 琛ㄧず錛氣�滆繖縐嶆柟娉曟湁娼滃姏褰誨簳鏀瑰彉鎴戜滑媯�(gè)嫻嬩漢綾昏泲鐧借川鐨勬柟寮忋�傚埄鐢ㄥ叏鐞冭祫婧愬鎵�鏈変漢綾昏泲鐧借川榪涜鏍囧噯鍖栧畾閲忚绔嬩竴浜涙柊鏍囧噯錛屾棤鐤戝皢鑳芥彁楂樹復(fù)搴婄爺絀剁殑鍙噸鐜版�э紝鍏跺皢瀵硅漿鍖栨柊鍨嬫不鐤楀拰璇婃柇甯︽潵宸ㄥぇ鐨勫獎(jiǎng)鍝嶃�傗�?/span>

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銆�銆�姣忎釜鏈夊墠鏅殑鐢熺墿鏍囪鐗╅兘蹇呴』鍦ㄤ復(fù)搴婅瘯楠屼腑寮�灞曡繘涓�姝ョ殑鐮旂┒錛岃繖灝辮姹傜爺絀朵漢鍛樿兘澶熸嫻嬫暟鐧懼埌鏁板崈涓?zhèn)ｈ�呮牱鏈腑姣忎釜鍊欓�夋爣蹇楃墿鐨勪赴搴︺�傜敱浜庡皢浠諱綍涓�縐嶅�欓�夋爣璁扮墿杞寲鑷充復(fù)搴婂簲鐢ㄧ殑鏈虹巼閮芥瀬鍏剁殑浣庯紝閴村埆涓�縐嶅叿鏈変復(fù)搴婁環(huán)鍊肩殑鐢熺墿鏍囪鐗╁繀欏誨澶ч噺鐨勮泲鐧借川榪涜嫻嬭瘯銆?/span>

銆�銆�Paulovich 琛ㄧず錛氣�滅幇鍦紝浣犺繕涓嶈兘瀵瑰ぇ澶氭暟鐨勪漢綾昏泲鐧借川榪涜澶ц妯℃嫻嬨�傚湪鎴戜滑瀹屾垚浜虹被鍩哄洜緇勬祴搴忥紝鑾峰緱DNA鍒嗗瓙鍏ㄧ洰褰?0澶氬勾涔嬪悗錛屼粛鐒朵笉鑳藉閲囩敤涓�縐嶆爣鍑嗗寲瀹氶噺鏂規(guī)硶鍦ㄥ悇縐嶉�氶噺妯″紡涓嬪浜虹被铔嬬櫧璐ㄧ粍寮�灞曠爺絀躲�傗�?/span>

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銆�銆�閲囩敤鐮旂┒浜哄憳寮�鍙戠殑榪欑鏂規(guī)硶錛屾瘡澶╂瘡鍙頒華鍣ㄨ兘澶熷鏈�灝?20 涓復(fù)搴婃牱鏈腑鐨?170 縐嶈泲鐧借川榪涜楂樺害鐗瑰紓鎬у湴銆佺簿紜湴銆佸璺畾閲忓垎鏋?浠諱綍鍏朵粬鐨勭幇鏈夋妧鏈兘娌℃湁榪欑鑳藉姏銆?/span>

銆�銆�鐢變簬璐ㄨ氨鎶�鏈槸閽堝鎬х殑錛岃繖鎰忓懗鐫�鐮旂┒浜哄憳鑳藉璋冩暣璁懼瀵繪壘鐧岀粏鑳?yōu)鎴栧叾浠栨牱鍝伨c誨瀷涓壒孌婄殑铔嬬櫧璐ㄤ簹緹わ紝鐩告瘮浜庨潪閽堝鎬х瓥鐣ワ紝瀹冨彲浠ュ湪鏇翠綆鐨勬按騫充笂媯�(gè)嫻嬪井閲忚娑叉牱鏈垨媧繪鏍囨湰涓洰鐨勮泲鐧借川鐨勫瓨鍦ㄣ�?/span>

銆�銆�鐮旂┒鐨勪富瑕佷綔鑰呫�丳aulovich 瀹為獙瀹ゅ垎鏋愬寲瀛﹀ Jacob Kennedy 璇達(dá)細(xì)鈥滄垜浠殑鐩爣鏄敤榪欎竴鎶�鏈潵鍙栦唬褰撳墠閲囩敤鐨勪竴浜涢潪甯歌�佹棫鐨勬妧鏈�傗�?/span>

銆�銆�褰撳墠錛岀爺絀朵漢鍛橀�氬父鏄噰鐢?Western blotting銆丒LISA 鎴栨槸鍏嶇柅緇勫寲(IHC)鎶�鏈潵媯�(gè)嫻嬩復(fù)搴婃牱鏈腑鐨勮泲鐧借川姘村鉤銆傝繖浜涙柟娉曞線寰�鏃犳硶鍦ㄥ疄楠屽涔嬮棿閲嶇幇緇撴灉錛屼粠鑰屽緢闅鵑獙璇侀�傜敤浜庝復(fù)搴婄殑鍊欓�夌敓鐗╂爣璁扮墿錛屽畠浠笉閫傜敤浜庝竴嬈℃嫻嬪ぇ閲忕殑铔嬬櫧璐ㄥ拰鏍鋒湰銆?/span>

銆�銆�Paulovich 鍜屽悓浜嬩滑閫氳繃鍒嗘瀽涔寵吅鐧岀粏鑳?yōu)鐢熸垚鐨?300 澶氱宸茬煡铔嬬櫧璐ㄩ獙璇佷簡(jiǎn)浠栦滑鐨勬妧鏈?鐮旂┒緇撴灉琛ㄦ槑錛孧RM-MS 鍙互閲嶇幇鍙?qiáng)鎵╁睍浠ュ線閲囩敤鍏朵粬鎶�鏈繘琛屼鉤鑵虹檶鐮旂┒鎵�鐢熸垚鐨勮瀵熺粨鏋溿�?/span>

銆�銆�璇ョ爺絀惰瘉瀹炰簡(jiǎn)錛孧RM-MS 鑳藉浠ヤ竴縐嶆爣鍑嗗寲鏂瑰紡涓�嬈℃嫻嬭澶氱殑铔嬬櫧璐紝涓哄紑灞曞浗闄呮�х殑銆佹湁緇勭粐鐨勭爺絀跺伐浣滃畾閲忎漢綾昏泲鐧借川涓殑姣忕铔嬬櫧濂犲畾浜?jiǎn)鍩杭媭銆?/span>
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