Calcium-dependent Hydrolysis of Supported Planar Lipids Were Triggered by honey bee venom Phospholipase A2 with Right Orientation at Interface
作者：Siqi Kai, Xu Li, Bolin Li, Xiaofeng Han, Xiaolin Lu*
關(guān)鍵字：PLA2 orientation, Ca2+, Lipids, SFG, LSCM
論文來(lái)源：期刊
具體來(lái)源：Phys. Chem. Chem. Phys. 2018, 20, 63-67
發(fā)表時(shí)間：2018年
我們結(jié)合三種光學(xué)技術(shù)解析了界面上磷脂酶水解磷脂分子的過(guò)程，看到了水解的動(dòng)力學(xué)過(guò)程以及水解產(chǎn)物在界面上的分布。其中，證實(shí)了酶的取向?qū)τ诮缑嫠獾墓δ苤陵P(guān)重要。這對(duì)于我們后續(xù)的研究具有直接的指導(dǎo)意義，我們的后續(xù)研究已經(jīng)開(kāi)展！
This paper was published as a back cover one in PCCP.
We combined sum frequency generation (SFG) vibrational spectroscopy, laser scanning confocal microscopy (LSCM) and attenuated total reflection infrared spectroscopy (ATR-IR) to study the hydrolysis of phospholipids catalyzed by a Ca2+-dependent enzyme, honey bee venom phospholipase A2 (bvPLA2). The experiments demonstrated that Ca2+ ions mediated between the lipids and bvPLA2, induced reorientation of bvPLA2, and activated hydrolysis. One of hydrolysis products, fatty acids, was desorbed, and the other one, lysophospholipids, self-organized at the interface. This study provides a refreshing and vivid picture at the molecular and micron levels for people to understand the Ca2+-dependent interfacial lipid hydrolysis catalyzed by bvPLA2.
http://pubs.rsc.org/en/content/articlehtml/2017/cp/c7cp06344j