[Colloids and Surfaces B: Biointerfaces] Single cell migration dynamics mediated by geometric confinement
作者：Hua Zhang, Ruixia Hou, Peng Xiao, Rubo Xing, Tao Chen, Yanchun Han,Penggang Ren, Jun Fu*
關(guān)鍵字：Graphene oxide micropatterns, Osteoblasts, Cell migration
論文來(lái)源：期刊
具體來(lái)源：Colloids and Surfaces B: Biointerfaces, 2016, 145, 72–78
發(fā)表時(shí)間：2016年
tThe migration dynamics of cells plays a key role in tissue engineering and regenerative medicine. Previousstudies mostly focus on regulating stem cell fate and phenotype by biophysical cues. In contrast, lessis known about how the geometric cues mediate the migration dynamics of cells. Here, we fabricategraphene oxide (GO) microstripes on cell non-adhesive PEG substrate by using micromolding in capillary(MIMIC) method. Such micropatterns with alternating cell adhesion and cell resistance enable an effectivecontrol of selective adhesion and migration of single cells. The sharp contrast in cell adhesion minimizesthe invasion of cells into the PEG patterns, and thereby strongly confines the cells on GO microstripes.As a result, the cells are forced to adapt highly polarized, elongated, and oriented geometry to fit thepatterns. A series of pattern widths have been fabricated to modulate the extent of cell deformation andpolarization. Under strong confinement, the cytoskeleton contractility, intracellular traction, and actinfilament elongation are highly promoted, which result in enhanced cell migration along the patterns.This work provides an important insight into developing combinatorial graphene-based patterns for thecontrol of cell migration dynamics, which is of great significance for tissue engineering and regenerativemedicine.