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JACS--Semisynthetic tellurosubtilisin with glutathione peroxidase activity
作者:Mao, SZ; Dong, ZY; Liu, JQ; et al.
關(guān)鍵字:Tellurosubtilisin, Glutathione peroxidase
論文來源:期刊
發(fā)表時(shí)間:2005年
Reaction of the hydroxyl group of serine-221 of subtilisin with phenylmethanesulfonylfluoride followed by nucleophilic substitution with sodium hydrogen telluride, a semisynthetic telluroprotein, tellurosubtilisin, was prepared. Tellurosubtilisin, which displays high substrate specificity for aromatic thiols, exhibits remarkable peroxidase activity and catalyzes the reduction of hydrogen peroxide by 3-carboxy-4-nitrobenzenethiol 20?000 times more efficiently than diphenyl diselenide.