Nanoscale Research Letters 2010: Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors
作者：Zhang M, Xue YN, Zhuo RX, Huang SW*
關鍵字：Gene delivery, Polycationic micelles, Biocleavable
論文來源：期刊
具體來源：Nanoscale Research Letters, 2010, 5, 1804-1811.
發(fā)表時間：2010年
An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N′-bis(acryloyl)cystamine with 90% yield. The polycationic micelles (198 nm, 32.5 mV), prepared from the amphiphilic polyamidoamine by dialysis method, can condense foreign plasmid DNA to form nanosized polycationic micelles/DNA polyelectrolyte complexes with positive charges, which transfected 293T cells with high efficiency. Under optimized conditions, the transfection efficiencies of polycationic micelles/DNA complexes are comparable to, or even higher than that of commercially available branched PEI (Mw 25 kDa).