Facile preparation of naphthol AS-based fluorescent probe for highly selective detection of cysteine in aqueous solution and its imaging application in living cells.
作者：Wei-Wei Ma, Meng-Yuan Wang, Di Yin, Xuan Zhang*.
關(guān)鍵字：fluorescent probe, cysteine, living cells imaging
論文來源：期刊
具體來源：Sens. Actuator B, 2017, 248, 332–337.
發(fā)表時間：2017年
tA simple and new fluorescent probe (1) for selective detection of cysteine (Cys) in aqueous solution wasfacilely synthesized. It was developed based on the masking the phenolic OH group in naphthol AS by amethacrylate group, where the methacrylate group acts as an electron acceptor to quench fluorescenceof naphthol AS as well as a reactive site for Cys. The conjugate addition/cyclization reaction of Cys towardmethacrylate moiety in the probe 1 led to a cleavage of the methacrylate group and release of freenaphthol AS, thereby induced a significant enhancement of fluorescence. The present fluorescent probeexhibited high selectivity toward Cys over homocysteine (Hcy) and glutathione (GSH) and worked wellin a physiological aqueous medium, allowing a successful application in living cells imaging.