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?/span>?/span>q?/span>?/span>?/span>开?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>一?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Ȁ?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>p?/span>l?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>往往?/span>?/span>J?/span>?/span>Q?/span>?/span>l?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>Q?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Ȁ?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>N-?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>Fmoc-FFQ?/span>?/span>一U?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>一c?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>pH?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>l?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>


?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>U?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>l?/span>?/span>一?/span>?/span>Fmoc-FFQ?/span>?/span>U?/span>c?/span>?/span>Q?/span>AuNCsQ?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>Q?/span>HRPQ?/span>?/span>?/span>?/span>ITO?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>Fmoc-FF/AuNCs/HRP?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>pH?/span>Cu(II)?/span>EDTA?/span>H2O2?/span>?/span>?/span>?/span>?/span>?/span>?/span>{?/span>?/span>Ȁ?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>U?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Constructing a Facile Biocomputing Platform Based on Smart Supramolecular Hydrogel Film Electrodes with Immobilized Enzymes and Gold Nanoclusters?/span>?/span>?/span>?/span>ACS Applied Materials & Interfaces?/span>?/span>?/span>?/span>?/span>W?/span>一?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>I?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>U?/span>?/span>?/span>?/span>?/span>?/span>p?/span>?/span>?/span> 

?/span> 1. Fmoc-FF/AuNCs/HRP?/span>?/span>?/span> (A) pH 9.0~?/span>?/span>?/span>?/span>, (B) ?/span>?/span>EDTA?/span>pH 5.0~?/span>?/span>?/span>?/span>, (C) ?/span>?/span>Cu(II)?/span>pH 5.0~?/span>?/span>?/span>?/span>?/span>?/span>l?/span>?/span>?/span>?/span>?/span>?/span>?/span>C?/span>?/span>?/span>?/span>


?/span>?/span>?/span>?/span>?/span>?/span>l?/span>?/span>?/span>?/span>?/span>Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>Q?/span>开?/span>?/span>一?/span>4?/span>?/span>/7?/span>?/span>?/span>?/span>?/span>?/span>?/span>一p?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>一?/span>?/span>?/span>GullibilityQ?/span>ANYQ?/span>?/span>?/span>?/span>?/span>?/span>p?/span>l?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>1Q?/span>?/span>q?/span>?/span>?/span>?/span>l?/span>?/span>?/span>?/span>Q?/span>?/span>ITO?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>U?/span>c?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>l?/span>?/span>?/span>?/span>Fmoc-FF?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>U?/span>?/span>Ȁ?/span>?/span>?/span>?/span>?/span>2Q?/span>l?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>p?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>Q?/span>?/span>?/span>?/span>Q?/span>FLQ?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>CVQ?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>UV-visQ?/span>?/span>?/span>?/span>?/span>3U?/span>c?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>p?/span>l?/span>?/span>?/span>?/span>?/span>?/span>3Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>p?/span>?/span>?/span>?/span>?/span>?/span>?/span>GullibilityQ?/span>ANYQ?/span>?/span>?/span>?/span>?/span>4Q?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>pH?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>U?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>q?/span>E?/span>?/span>?/span>HRP?/span>?/span>?/span>?/span>?/span>?/span>Cu(II)-?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>一q?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>所?/span>?/span>?/span>?/span>?/span>?/span>p?/span>l?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>一q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>I?/span>?/span>?/span>?/span>?/span>?/span>?/span>D?/span>Q?/span>?/span>?/span>?/span>q?/span>一{?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>l?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>/?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>{?/span>?/span>?/span>?/span>?/span>?/span>?/span>

?/span> 2. ?/span>?/span>?/span>?/span>?/span>?/span>(Fmoc-FF)?/span>?/span>?/span>?/span>?/span>(Fmoc-FF-)?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>



?/span> 3. (A) ?/span>365 nm?/span>?/span>?/span>Ȁ?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>(a) Fmoc-FF; (b) Fmoc-FF/AuNCs; (c) Fmoc-FF/AuNCs/HRP; (d) ITO/PDDA?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>?/span>?/span>?/span>(?/span>) ?/span>?/span>?/span>; (?/span>) 365 nm?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(B) ?/span>?/span>-?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>(a) 0.2 mg/mL?/span>HRP?/span>?/span>Q?/span>?/span>?/span>pH 7.0~?/span>?/span>?/span>?/span>Q?/span>; (b) Fmoc-FF/AuNCs/HRP; (c) Fmoc-FF; (d) Fmoc-FF/AuNCs?/span>?/span>?/span>?/span>?/span>



?/span> 4. Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>SEM?/span>?/span>Q?/span>(A) pH 5.0Q?/span>(B) pH 5.0 Cu(II)Q?/span>(C) pH 5.0 H2O2Q?/span>(D) pH 9.0Q?/span>(E) pH 9.0 Cu(II)Q?/span>(F) pH 9.0 H2O2?/span>


?/span> 5. (A) Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>(a) pH 5.0?/span>(b) pH 9.0~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>FL660?/span>pH?/span>?/span>?/span>?/span>(B) Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>?/span>?/span>?/span>FL660?/span>pH 5.0?/span>pH 9.0~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(C) Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Cu(II)?/span>pH 5.0~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>FL660?/span>Cu(II)?/span>?/span>?/span>?/span>?/span>?/span>(D) Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>?/span>?/span>?/span>FL660?/span>?/span>?/span>?/span>?/span>1.8 mM Cu(II)?/span>3.6 mM EDTA?/span>pH 5.0~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(E) Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>H2O2?/span>pH 5.0~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>FL660?/span>H2O2?/span>?/span>?/span>?/span>?/span>?/span>(F) ?/span>?/span>?/span>?/span>?/span>?/span>FL660?/span>?/span>?/span>(a) 0 mM H2O2?/span>(b) 2.0 mM H2O2~?/span>?/span>?/span>?/span>?/span>?/span>pH?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>



?/span> 6. (A) ?/span>-0.35 V?/span>?/span>?/span>?/span>Q?/span>?/span>pH 9.0 (a?/span>c)?/span>pH 5.0 (b?/span>d)~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>0.4 mM H2O2?/span>Q?/span>Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>(a?/span>bQ?/span>?/span>U?/span>)?/span>ITO?/span>?/span>?/span>(c?/span>dQ?/span>?/span>U?/span>)?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(B) ?/span>-0.35 V?/span>?/span>?/span>?/span>Q?/span>Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>pH?/span>?/span>?/span>?/span>?/span>?/span>?/span>Q?/span>?/span>-0.35 V?/span>?/span>?/span>?/span>Q?/span>Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>pH 5.0?/span>pH 9.0~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(C)?/span>-0.35 V?/span>?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>(a)?/span>?/span>?/span>?/span>(b)?/span>?/span>1.8 mM Cu(II)?/span>?/span>(c)?/span>?/span>1.8 mM Cu(II)?/span>3.6 mM EDTA?/span>pH 5.0 ~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>0.4 mM H2O2?/span>Q?/span>Fmoc-FF/AuNCs/HRP ?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(D) ?/span>-0.35 V?/span>?/span>?/span>?/span>Q?/span>Fmoc-FF/AuNCs/HRP?/span>?/span>?/span>?/span>?/span>?/span>?/span>1.8 mM Cu(II)?/span>3.6 mM EDTA?/span>pH 5.0~?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>



?/span> 7. ?/span>?/span>p?/span>?/span>16U?/span>?/span>?/span>l?/span>?/span>?/span>?/span>(A)?/span>?/span>?/span>?/span>(FL660)Q?/span>(B)?/span>?/span>-?/span>?/span>?/span>?/span>?/span>?/span>(E730)?/span>(C)?/span>-0.35 V?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(?I?)?/span>?/span>?/span>?/span>?/span>?/span>U?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(D) 4?/span>?/span>/7?/span>?/span>?/span>?/span>|?/span>l?/span>?/span>W?/span>?/span>C?/span>?/span>?/span>?/span>


?/span> 8. (A) ?/span>q?/span>?/span>GullibilityQ?/span>ANYQ?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>(B) ?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>9U?/span>?/span>?/span>l?/span>?/span>?/span>Q?/span>?/span>?/span>p?/span>?/span>?/span>?/span>?/span>?/span>(FL660)?/span>?/span>?/span>?/span>?/span>?/span>U?/span>?/span>?/span>Q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>q?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>?/span>



?/strong>?/strong>?/strong>?/strong>

Li, J.; Xiao, C.; Wei, W.; Xiao, R.; Yao, H.; Liu, H. Constructing a Facile Biocomputing Platform Based on Smart Supramolecular Hydrogel Film Electrodes with Immobilized Enzymes and Gold Nanoclusters. ACS Appl. Mater. Interfaces 2021.

https://pubs.acs.org/doi/10.1021/acsami.1c11206



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